8VM2
Crystal Structure of NRAS Q61K bound to GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-03 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1.542 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 75.789, 55.699, 149.291 |
| Unit cell angles | 90.00, 98.15, 90.00 |
Refinement procedure
| Resolution | 29.740 - 1.740 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.069 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.740 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.056 | 0.749 |
| Rmeas | 0.065 | 0.975 |
| Rpim | 0.032 | 0.616 |
| Total number of observations | 234839 | 7765 |
| Number of reflections | 63393 | 3430 |
| <I/σ(I)> | 15.8 | 1.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.7 | 2.3 |
| CC(1/2) | 0.998 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 298 | PEG/Ion (containing 0.06 M Citric acid, 0.04 M BIS-TRIS propane)/pH 4.1, 16% w/v Polyethylene glycol 3350 |
