8VEU
Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 23
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97779 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 103.370, 138.790, 179.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.800 - 2.460 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.25027 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.550 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.078 | 0.934 |
| Rmeas | 0.085 | 1.013 |
| Rpim | 0.033 | 0.390 |
| Total number of observations | 30741 | |
| Number of reflections | 47213 | 4610 |
| <I/σ(I)> | 14.5 | 2.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.999 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0) |
