8UDC
Crystal structure of TcPINK1 in complex with CYC116
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 53.021, 53.021, 546.158 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.920 - 3.100 |
R-factor | 0.2585 |
Rwork | 0.255 |
R-free | 0.32930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.558 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.920 | 3.310 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.850 | |
Number of reflections | 9497 | 1611 |
<I/σ(I)> | 7.8 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 35.3 | 38.6 |
CC(1/2) | 0.996 | 0.532 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 90 mM HEPES 7.0, 17.5% PEG 4K, 0.13 M Ammonium sulfate |