8TZX
Ternary complex structure of Cereblon-DDB1 bound to WIZ(ZF7) and the molecular glue dWIZ-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-05-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.669, 137.742, 136.628 |
Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
Resolution | 39.310 - 3.150 |
R-factor | 0.2152 |
Rwork | 0.213 |
R-free | 0.25190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.518 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.390 | 3.250 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.056 | 1.028 |
Rmeas | 0.275 | 4.948 |
Rpim | 0.074 | 1.325 |
Total number of observations | 677068 | 62023 |
Number of reflections | 48824 | 4468 |
<I/σ(I)> | 7.2 | 0.7 |
Completeness [%] | 100.0 | |
Redundancy | 13.9 | 13.9 |
CC(1/2) | 0.997 | 0.380 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M Sodium Sulfate, 0.1 M Bis-Tris Propane pH 7.5, 20% w/v PEG 3350 |