8TZX

Ternary complex structure of Cereblon-DDB1 bound to WIZ(ZF7) and the molecular glue dWIZ-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2020-05-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	76.669, 137.742, 136.628
Unit cell angles	90.00, 96.92, 90.00

Refinement procedure

Resolution	39.310 - 3.150
R-factor	0.2152
Rwork	0.213
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.518
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.390	3.250
High resolution limit [Å]	3.150	3.150
Rmerge	0.056	1.028
Rmeas	0.275	4.948
Rpim	0.074	1.325
Total number of observations	677068	62023
Number of reflections	48824	4468
<I/σ(I)>	7.2	0.7
Completeness [%]	100.0
Redundancy	13.9	13.9
CC(1/2)	0.997	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2 M Sodium Sulfate, 0.1 M Bis-Tris Propane pH 7.5, 20% w/v PEG 3350