8TTA
Structure of retromer VPS29-VPS35 (483-796) complexed with Fam21A repeat 21 (1328-1341)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.953700 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.730, 165.577, 68.000 |
Unit cell angles | 90.00, 117.51, 90.00 |
Refinement procedure
Resolution | 48.750 - 3.460 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.075 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.310 | 3.790 |
High resolution limit [Å] | 3.460 | 3.460 |
Rmerge | 0.342 | 1.233 |
Rmeas | 0.370 | 1.330 |
Rpim | 0.139 | 0.494 |
Total number of observations | 110783 | 26173 |
Number of reflections | 15793 | 3693 |
<I/σ(I)> | 4.5 | 1.3 |
Completeness [%] | 99.3 | |
Redundancy | 7 | 7.1 |
CC(1/2) | 0.993 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M MES pH 6.5, 0.1 M Magnesium Acetate, 10% PEG10,000 |