8TS0

Crystal Structure of human ASGR1 CRD (Carbohydrate Recognition Domain) bound to 8M24 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	38.911, 90.316, 167.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.047 - 1.700
R-factor	0.1743
Rwork	0.173
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14rc1_3177)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.910	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.103	2.814
Rmeas	0.107	2.923
Rpim	0.030	0.789
Total number of observations	863662	46748
Number of reflections	66288	3439
<I/σ(I)>	14.7	0.9
Completeness [%]	100.0
Redundancy	13	13.6
CC(1/2)	0.999	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	100mM SPG (succinic acid:sodium dihydrogen phosphate:glycine) buffer pH 9.0, 25% PEG1500, Cryo protectant: 20% glycerol