8TMU

HLA-B*73:01 bound to a 10mer peptide in complex with KIR2DL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03320
Spacegroup name	P 43 21 2
Unit cell lengths	92.690, 92.690, 200.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.300 - 2.900
R-factor	0.2177
Rwork	0.215
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.550
Data reduction software	iMOSFLM (7.4.0)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (1.20.1_4487+SVN)
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.300	3.004
High resolution limit [Å]	2.900	2.900
Number of reflections	20180	1973
<I/σ(I)>	11.9
Completeness [%]	100.0	100
Redundancy	16.8
CC(1/2)	1.000	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris-HCl pH 8.75 and 20% PEG 8000