8TH1
Crystal Structure of the G3BP1 NTF2-like domain bound to the IDR1 of SARS-CoV-2 nucleocapsid protein D3L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.880, 85.890, 70.690 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 48.720 - 1.800 |
| R-factor | 0.20009 |
| Rwork | 0.198 |
| R-free | 0.23828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q90 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.608 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.720 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 1.106 | |
| Number of reflections | 51633 | 3223 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.956 | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M sodium thiocyanate, 20% PEG 3350 |
