8TBQ
The crystal structure of human VISTA extra cellular domain in complex with Fab fragment of pH-selective anti-VISTA antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-14 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9998 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 207.659, 39.512, 177.979 |
Unit cell angles | 90.00, 117.12, 90.00 |
Refinement procedure
Resolution | 158.409 - 2.593 |
Rwork | 0.237 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.941 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 158.409 | 2.837 |
High resolution limit [Å] | 2.593 | 2.593 |
Number of reflections | 26185 | 1309 |
<I/σ(I)> | 8.9 | |
Completeness [%] | 84.6 | 52.7 |
Redundancy | 4.27 | 4.58 |
CC(1/2) | 0.996 | 0.695 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M BIS-TRIS propane, 0.2 M NaBr, 22%PEG3350 |