8T2D
Ubiquitin variant i53:Mutant T12Y.T14E.L67R with 53BP1 Tudor domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97939 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.511, 46.855, 91.122 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.850 - 1.751 |
Rwork | 0.222 |
R-free | 0.27250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.348 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.850 | 1.813 |
High resolution limit [Å] | 1.751 | 1.751 |
Rmerge | 0.060 | |
Rmeas | 0.065 | |
Rpim | 0.025 | 0.937 |
Number of reflections | 17557 | 1636 |
<I/σ(I)> | 12.41 | |
Completeness [%] | 98.9 | 94.24 |
Redundancy | 6.6 | 3.8 |
CC(1/2) | 0.999 | 0.386 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M MES pH 6.0, 0.2 M Trimethylamine N-oxide dehydrate, 13% w/v PEG MME 2000 |