8SX4

Crystal Structure of eIF4e in complex with Compound 7n

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1276
Spacegroup name	P 1 21 1
Unit cell lengths	49.125, 74.328, 51.877
Unit cell angles	90.00, 97.13, 90.00

Refinement procedure

Resolution	37.810 - 1.986
R-factor	0.1998
Rwork	0.197
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	BUSTER (2.10.4 (17-FEB-2023))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	1.986	5.430	2.000
Rmerge	0.074	0.043	0.383
Rmeas	0.083	0.046	0.436
Rpim	0.035	0.018	0.204
Number of reflections	24897	1315	1245
<I/σ(I)>	10.1
Completeness [%]	98.0	99.8	99.2
Redundancy	5.2	6.5	4.2
CC(1/2)	0.987	0.998	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16-26% Peg 3350, 0.1 M MES pH 6.0, 10% isopropanol and 2 mM CaCl2