8SVE

Structure of Monomeric Interleukin-10 Grafted into and Antibody CDR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 1 21 1
Unit cell lengths	80.641, 104.662, 82.826
Unit cell angles	90.00, 115.29, 90.00

Refinement procedure

Resolution	42.900 - 2.400
R-factor	0.1906
Rwork	0.188
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.855
Data reduction software	DENZO (2.3.10)
Data scaling software	SCALEPACK (2.3.10)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.122	0.794
Rmeas	0.145	0.953
Rpim	0.077	0.521
Number of reflections	47411	3890
<I/σ(I)>	9.4	1.12
Completeness [%]	96.9	79.9
Redundancy	3.3	2.9
CC(1/2)		0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	294	20% PEG 3350, 0.2M Magnesium Acetate, pH 7.9