8SDG

Crystal structure of SARS-CoV-2 receptor binding domain in complex with neutralizing antibody CC25.43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	311.948, 83.786, 74.150
Unit cell angles	90.00, 100.80, 90.00

Refinement procedure

Resolution	72.840 - 2.710
R-factor	0.2572
Rwork	0.255
R-free	0.30760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.697
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.213	2.760
High resolution limit [Å]	2.710	2.710
Number of reflections	47774	2451
<I/σ(I)>	7.8
Completeness [%]	93.4
Redundancy	5.2
CC(1/2)	0.995	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1.0 M Li-chloride, 10% PEG-6000, and 0.1 M citric acid pH 4.0