8S7C

Ternary Complex of Cachd1, FZD5 and LRP6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	283.697, 198.236, 218.824
Unit cell angles	90.00, 128.07, 90.00

Refinement procedure

Resolution	86.130 - 4.700
R-factor	0.2092
Rwork	0.206
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.577
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	172.259	5.400
High resolution limit [Å]	4.700	4.700
Number of reflections	23514	1177
<I/σ(I)>	6.3
Completeness [%]	88.8
Redundancy	6.8
CC(1/2)	0.990	0.437

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	296	0.1 M Calcium Acetate 0.1 M Sodium Acetate 10% PEG 4000 pH 4.5