8RP8
Structure of K2 Fab in complex with human CD47 ECD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.415, 71.812, 78.379 |
| Unit cell angles | 109.45, 95.55, 118.90 |
Refinement procedure
| Resolution | 20.060 - 2.000 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.220 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.036 | 0.145 |
| Rmeas | 0.043 | 0.171 |
| Rpim | 0.022 | 0.089 |
| Number of reflections | 78163 | 4427 |
| <I/σ(I)> | 19.2 | 7.5 |
| Completeness [%] | 97.4 | 96.8 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.999 | 0.981 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M sodium citrate, 20% PEG 8000 |
