8RDS

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with spiro-isoxazol based compound 8i

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-23
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0001
Spacegroup name	P 21 21 21
Unit cell lengths	56.385, 59.297, 86.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.860 - 2.000
R-factor	0.20883
Rwork	0.207
R-free	0.25337
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.413
Data reduction software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.860	48.860	2.120
High resolution limit [Å]	2.000	5.960	2.000
Rmeas	0.103	0.044	1.572
Number of reflections	37772	1429	6135
<I/σ(I)>	10.65	36.48	1.11
Completeness [%]	100.0	99.8	99.9
Redundancy	7.02	6.73	7.1
CC(1/2)	0.999	0.998	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3 mM thalidomide