8PW8

Crystal structure of the human METTL3-METTL14 in complex with a bisubstrate analogue (BA2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	63.780, 63.780, 225.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.510 - 2.300
R-factor	0.1988
Rwork	0.196
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	24709	3866
<I/σ(I)>	15.74
Completeness [%]	99.8
Redundancy	8.1
CC(1/2)	0.990	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 0.4M Magnesium acetate