8PJE
Human Leukocyte Antigen class II allotype DR1 presenting influenza A virus haemagglutinin (HA)306-318 PKYVKQNTLKLAT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.500, 89.180, 137.440 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 32.810 - 1.700 |
| R-factor | 0.1724 |
| Rwork | 0.171 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.639 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.810 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.083 | 0.884 |
| Rmeas | 0.097 | 1.039 |
| Rpim | 0.049 | 0.539 |
| Number of reflections | 109380 | 10898 |
| <I/σ(I)> | 9.48 | 1.36 |
| Completeness [%] | 99.5 | 99.55 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.997 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M HEPES pH 7.0, 25 % PEG8000, 15 % Glycerol |
