8PDE

Crystal Structure of the MADS-box/MEF2 Domain of MEF2D bound to dsDNA and HDAC4 deacetylase binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-11
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	57.024, 76.330, 56.240
Unit cell angles	90.00, 92.57, 90.00

Refinement procedure

Resolution	45.650 - 2.400
Rwork	0.217
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.650	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.132	0.865
Number of reflections	17911	1938
<I/σ(I)>	7	1.7
Completeness [%]	94.7
Redundancy	4.1
CC(1/2)	0.992	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium formate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350