8PC2
SelDeg51 in complex with FKBP51FK1 domain and pVHL:EloB:EloC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-01-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.918400 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 138.646, 68.425, 159.256 |
Unit cell angles | 90.00, 114.45, 90.00 |
Refinement procedure
Resolution | 48.739 - 2.800 |
Rwork | 0.210 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.581 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.740 | 48.740 | 2.940 |
High resolution limit [Å] | 2.800 | 9.290 | 2.800 |
Rmerge | 0.088 | 0.028 | 0.523 |
Rmeas | 0.113 | 0.036 | 0.668 |
Rpim | 0.070 | 0.022 | 0.409 |
Number of reflections | 33478 | 946 | 4459 |
<I/σ(I)> | 12 | ||
Completeness [%] | 99.1 | ||
Redundancy | 4.6 | 4 | 4.7 |
CC(1/2) | 0.997 | 0.999 | 0.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 15% PEG3350, 0.2 M tri-sodium citrate, 0.1 M HEPES-NaOH pH 7.5 and 10% glycol |