8OXU
Crystal Structure of the Hsp90-LA1011 Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96864 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 97.714, 97.714, 275.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.710 - 2.940 |
R-factor | 0.2498 |
Rwork | 0.247 |
R-free | 0.30620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.051 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.100 | 3.190 |
High resolution limit [Å] | 2.940 | 2.940 |
Number of reflections | 23054 | 1153 |
<I/σ(I)> | 9 | 1.4 |
Completeness [%] | 91.9 | 58.1 |
Redundancy | 9.2 | 4 |
CC(1/2) | 0.995 | 0.361 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | MES pH 7.5, Magnesium chloride hexahydrate, PEG Smear Medium, 2propanol |