8OS5

Crystal structure of the Factor XII heavy chain reveals an interlocking dimer with a FnII to kringle domain interaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	I 21 21 21
Unit cell lengths	144.536, 144.594, 155.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.970 - 3.400
R-factor	0.2616
Rwork	0.255
R-free	0.31880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.535
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.970	3.716
High resolution limit [Å]	3.394	3.394
Number of reflections	14002	701
<I/σ(I)>	7.1
Completeness [%]	94.2
Redundancy	6.4
CC(1/2)	0.994	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	5% PAA1200, 0.1M urea.