8OM6

Crystal structure of FGF2-STAB, a stable variant of human fibroblast growth factor 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	117.510, 38.670, 34.260
Unit cell angles	90.00, 104.64, 90.00

Refinement procedure

Resolution	33.148 - 1.310
R-factor	0.1982
Rwork	0.197
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fgf
RMSD bond length	0.005
RMSD bond angle	0.799
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.148	1.357
High resolution limit [Å]	1.310	1.310
Rmeas	0.043	1.216
Number of reflections	35777	3496
<I/σ(I)>	15.28	0.75
Completeness [%]	99.4
Redundancy	3.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	292.15	PEG 3350, Bis-Tris