8K4Q

Crystal structure of nanobody HuNb103 bound to human interleukin-4 receptor subunit alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-25
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	57.870, 109.598, 64.167
Unit cell angles	90.00, 98.48, 90.00

Refinement procedure

Resolution	57.300 - 2.590
R-factor	0.2249
Rwork	0.222
R-free	0.27987
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.600	2.660
High resolution limit [Å]	2.590	2.590
Rmerge	0.155	2.158
Rmeas	0.169	2.351
Rpim	0.066	0.925
Total number of observations	161738	11813
Number of reflections	24709	1843
<I/σ(I)>	9.7	1.2
Completeness [%]	100.0
Redundancy	6.5	6.4
CC(1/2)	0.994	0.412

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	Reservoir condition: 0.1M HEPES pH 7.0, 20% PEG8000. Sample buffer: 25mM HEPES pH 7.49, 150mM NaCl. Cryoprotectant condition: 0.1M HEPES pH 7.0, 20% PEG8000, 15% ethylene glycol.