8K4Q
Crystal structure of nanobody HuNb103 bound to human interleukin-4 receptor subunit alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-25 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.870, 109.598, 64.167 |
Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
Resolution | 57.300 - 2.590 |
R-factor | 0.2249 |
Rwork | 0.222 |
R-free | 0.27987 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.600 | 2.660 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.155 | 2.158 |
Rmeas | 0.169 | 2.351 |
Rpim | 0.066 | 0.925 |
Total number of observations | 161738 | 11813 |
Number of reflections | 24709 | 1843 |
<I/σ(I)> | 9.7 | 1.2 |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 0.994 | 0.412 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Reservoir condition: 0.1M HEPES pH 7.0, 20% PEG8000. Sample buffer: 25mM HEPES pH 7.49, 150mM NaCl. Cryoprotectant condition: 0.1M HEPES pH 7.0, 20% PEG8000, 15% ethylene glycol. |