8JI8
Crystal Structure of Prophenoloxidase PPO6 chimeric mutant (F215EASNRAIVD224 to G215DGPDSVVR223) from Aedes aegypti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.570, 153.660, 223.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.690 - 2.650 |
R-factor | 0.18018 |
Rwork | 0.176 |
R-free | 0.26133 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.415 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MR-Rosetta |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.690 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.160 | 0.790 |
Rmeas | 0.173 | 0.907 |
Rpim | 0.066 | 0.436 |
Number of reflections | 90756 | 3878 |
<I/σ(I)> | 12.2 | 2.1 |
Completeness [%] | 98.6 | 86.3 |
Redundancy | 12.6 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.1 M imidazole pH7.0, 2% v/v Tacsimate pH7.0, 5% v/v 2-ProOH, 6%-10% v/v PEG 3350, |