8JC5

Crystal structure of PLEKHM1 RUN domain in complex with GTP-bound Arl8b(Q75L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97873
Spacegroup name	P 1 21 1
Unit cell lengths	80.976, 73.817, 91.959
Unit cell angles	90.00, 114.18, 90.00

Refinement procedure

Resolution	46.750 - 2.010
R-factor	0.1686
Rwork	0.167
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.205
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.890	2.040
High resolution limit [Å]	2.010	2.010
Rmerge	0.047	0.573
Number of reflections	63813	63813
<I/σ(I)>	15.4
Completeness [%]	96.9
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Lithium chloride, 0.1 M TRIS pH=7.6, 18% (w/v) PEG 8000