8J6K

Crystal structure of pro-interleukin-18 and caspase-4 complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	86.254, 86.254, 194.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.200 - 3.120
R-factor	0.2411
Rwork	0.238
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.550
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.700	3.290
High resolution limit [Å]	3.120	3.120
Rmerge	0.113	1.312
Rmeas	0.119
Rpim	0.035	0.410
Number of reflections	15561	2223
<I/σ(I)>	16	2.6
Completeness [%]	100.0	100
Redundancy	11.1	11.2
CC(1/2)	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Calcium acetate, 0.1M Tris-HCl pH 9.0, 13% PEG 8000, 0.01 M L-Glutathione reduced, 0.01 M L-Glutathione oxidized