8IMU
Dihydroxyacid dehydratase (DHAD) mutant-V497F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-12 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 135.120, 135.120, 136.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 1.930 |
| R-factor | 0.1829 |
| Rwork | 0.182 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ze4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.108 | 1.473 |
| Number of reflections | 95022 | 4650 |
| <I/σ(I)> | 13.6 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.2 | 13.4 |
| CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 0.1 M sodium acetate pH 5.0 1.5 M ammonium sulfate |
