8IFO
Crystal structure of estrogen related receptor-gamma DNA binding domain complexed with Pla2g12b promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.403, 74.434, 111.020 |
| Unit cell angles | 90.00, 104.98, 90.00 |
Refinement procedure
| Resolution | 44.290 - 2.200 |
| R-factor | 0.22041 |
| Rwork | 0.219 |
| R-free | 0.26001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.651 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.620 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.084 | 0.509 |
| Rmeas | 0.091 | 0.557 |
| Rpim | 0.036 | 0.223 |
| Total number of observations | 199080 | 16364 |
| Number of reflections | 30806 | 2668 |
| <I/σ(I)> | 9.8 | 2.7 |
| Completeness [%] | 99.2 | |
| Redundancy | 6.5 | 6.1 |
| CC(1/2) | 0.994 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M Sodium malonate pH 6.0, 12% w/v Polyethylene glycol 3,350 |
