8ID2

Crystal structure of the ubiquitin-like domain in the SF3A1 subunit of human U2 snRNP complexed with the stem-loop 4 of U1 snRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.07000
Spacegroup name	P 21 21 21
Unit cell lengths	54.455, 60.214, 103.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.390 - 1.800
R-factor	0.1965
Rwork	0.194
R-free	0.23740
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.390	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.049	4.198
Rmeas	0.051	4.362
Rpim	0.014	1.604
Number of reflections	32309	1809
<I/σ(I)>	20.5
Completeness [%]	99.7	95.8
Redundancy	13.4	13.4
CC(1/2)	0.999	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM MIB buffer (pH 5.0), 25% PEG 1500