8HNW

Crystal structure of HpaCas9-sgRNA surveillance complex bound to double-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	158.867, 185.760, 160.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.620 - 3.410
R-factor	0.2588
Rwork	0.258
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hnt
RMSD bond length	0.003
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.400	3.400
Rmerge	0.132	1.691
Number of reflections	32947	1628
<I/σ(I)>	16.64
Completeness [%]	100.0
Redundancy	7.2
CC(1/2)	0.990	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	tris, sodium chloride, sodium acetate, lithium sulfate, PEG 10000