8HNW

Crystal structure of HpaCas9-sgRNA surveillance complex bound to double-stranded DNA

Summary for 8HNW

Entry DOI	10.2210/pdb8hnw/pdb
Descriptor	CRISPR-associated endonuclease Cas9, sgRNA, Target strand, ... (4 entities in total)
Functional Keywords	cas9, antimicrobial protein, dsdna, immune system
Biological source	Haemophilus parainfluenzae More
Total number of polymer chains	4
Total formula weight	176721.08
Authors	Sun, W.,Cheng, Z.,Wang, Y. (deposition date: 2022-12-08, release date: 2023-07-19, Last modification date: 2023-08-02)
Primary citation	Sun, W.,Cheng, Z.,Wang, J.,Yang, J.,Li, X.,Wang, J.,Chen, M.,Yang, X.,Sheng, G.,Lou, J.,Wang, Y. AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation. Proc.Natl.Acad.Sci.USA, 120:e2303675120-e2303675120, 2023 Cited by PubMed: 37494395 DOI: 10.1073/pnas.2303675120 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.41 Å)

Structure validation