8HNW
Crystal structure of HpaCas9-sgRNA surveillance complex bound to double-stranded DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1055 | 121649.7 | 1 | UniProt (F0ET08) | Haemophilus parainfluenzae | |
| 2 | B (B) | sgRNA | polymer | 128 | 40860.1 | 1 | synthetic construct | ||
| 3 | C (C) | Target strand | polymer | 35 | 10849.0 | 1 | Haemophilus parainfluenzae | ||
| 4 | D (D) | Non-target strand | polymer | 11 | 3362.2 | 1 | Haemophilus parainfluenzae |
Sequence modifications
A: 1 - 1054 (UniProt: F0ET08)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 581 | His 581 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 176721.1 | |
| All* | Total formula weight | 176721.1 |
