8HNT
Crystal structure of anti-CRISPR protein AcrIIC4 bound to HpaCas9-sgRNA surveillance complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.834, 134.592, 67.531 |
| Unit cell angles | 90.00, 105.04, 90.00 |
Refinement procedure
| Resolution | 33.220 - 3.060 |
| R-factor | 0.2381 |
| Rwork | 0.237 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jdq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.572 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.080 | 0.716 |
| Number of reflections | 32735 | 1572 |
| <I/σ(I)> | 12.95 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.996 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | tris, sodium chloride, PEG 3000 |
