8HCF

Crystal structure of mTREX1-UMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	64.031, 85.771, 100.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.143 - 1.600
R-factor	0.1543
Rwork	0.152
R-free	0.17890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yws
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.053	0.037	0.490
Rmeas	0.057	0.040	0.526
Rpim	0.020	0.014	0.191
Total number of observations	558283
Number of reflections	73263	7648	7223
<I/σ(I)>	15.8
Completeness [%]	99.9	99.2	100
Redundancy	7.6	7.3	7.3
CC(1/2)		0.999	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350