8GUW
Structure of Aurora Kinase A in complex with activator peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-05 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.859, 78.695, 185.634 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.980 - 2.700 |
R-factor | 0.2159 |
Rwork | 0.212 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dt3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.030 | 0.170 |
Number of reflections | 26742 | 2635 |
<I/σ(I)> | 16.625 | |
Completeness [%] | 99.6 | |
Redundancy | 9.4 | |
CC(1/2) | 0.991 | 0.693 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 291 | 0.1M Tris pH 8.0, 0.2M lithium sulfate, 15% (w/v) polyethylene glycol 3350 |