8GQ1

HyHEL10 Fab complexed with hen egg lysozyme carrying arginine cluster in framework region of light chain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	147.900, 147.900, 137.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.800 - 3.130
R-factor	0.2314
Rwork	0.230
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d9a
RMSD bond length	0.004
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800	3.240
High resolution limit [Å]	3.130	3.130
Rmerge	0.090	0.575
Rmeas	0.128	0.813
Rpim	0.090	0.575
Number of reflections	16124	1566
<I/σ(I)>	8.86	1.08
Completeness [%]	99.3	99.94
Redundancy	2	2
CC(1/2)	0.987	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG 400, sodium citrate tribasic dihydrate, Tris hydrochloride