8GHP
GUCY2C-ECD bound to anti-GUCY2C-scFv antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-09-25 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 199.629, 199.629, 123.267 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.210 - 3.520 |
R-factor | 0.275 |
Rwork | 0.274 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 3.770 |
High resolution limit [Å] | 3.520 | 3.520 |
Rmerge | 0.120 | 0.900 |
Number of reflections | 17636 | 881 |
<I/σ(I)> | 8.3 | |
Completeness [%] | 91.8 | |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2M ammonium H2-PO4, 100mM Tris hydrochloride at pH 8.5 |