8FXS
Crystal structure of human pro-TGF-beta2 in complex with Nb9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92009 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.683, 89.554, 89.740 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 48.270 - 3.150 |
| R-factor | 0.2546 |
| Rwork | 0.249 |
| R-free | 0.30090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data reduction software | XDS (20190417) |
| Data scaling software | XDS (20190417) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 3.263 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 18849 | 1881 |
| <I/σ(I)> | 8.48 | |
| Completeness [%] | 98.3 | 99.37 |
| Redundancy | 3.4 | 3.6 |
| CC(1/2) | 0.967 | 0.423 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Crystals of the Nb9/proTGF-beta2 complex (1 microliter) were formed in hanging drops with 1 microliter of 100 mM HEPES pH 7.6, 10% PEG 4000. |
