8FT8

The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Thr-Val linker variant, SUMO tag-free preparation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	P 65
Unit cell lengths	100.882, 100.882, 49.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.870 - 1.600
R-factor	0.1833
Rwork	0.182
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qar 1shu
RMSD bond length	0.006
RMSD bond angle	0.758
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.870	1.658
High resolution limit [Å]	1.600	1.600
Rmerge	0.056	0.990
Rmeas	0.058
Rpim	0.013	0.225
Number of reflections	38098	3766
<I/σ(I)>	25.28
Completeness [%]	99.6	99.13
Redundancy	19
CC(1/2)	0.999	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M HEPES, 2.0M ammonium sulfate