8F93

WDR5 covalently modified at Y228 by (R)-2-SF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	92.780, 103.022, 81.014
Unit cell angles	90.00, 101.55, 90.00

Refinement procedure

Resolution	32.260 - 2.300
R-factor	0.2055
Rwork	0.203
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.236
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.260	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.077	0.787
Number of reflections	32875	3285
<I/σ(I)>	9.2
Completeness [%]	99.0
Redundancy	3.3
CC(1/2)	0.997	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Ammonium sulfate, 0.1 M MES pH 6.5, 20% w/v PEG 8000