8EZL

Pfs25 in complex with transmission-reducing antibody AS01-50

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033132
Spacegroup name	C 1 2 1
Unit cell lengths	88.610, 55.750, 86.090
Unit cell angles	90.00, 103.63, 90.00

Refinement procedure

Resolution	46.800 - 2.300
R-factor	0.2195
Rwork	0.215
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6phb
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14.3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.382
High resolution limit [Å]	2.300	2.300
Rpim	0.037	0.301
Number of reflections	18026	1731
<I/σ(I)>	14.13	2.25
Completeness [%]	98.3	94.75
Redundancy	3.4	2.8
CC(1/2)	0.998	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M lithium chloride, 0.1M HEPES pH 7.5, and 25% PEG 6000