8EI3
Crystal structure of VHL in complex with H313, a Helicon Polypeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 |
Unit cell lengths | 47.080, 47.080, 359.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.080 - 3.490 |
R-factor | 0.2192 |
Rwork | 0.216 |
R-free | 0.28530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vcb |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.080 | 3.820 |
High resolution limit [Å] | 3.490 | 3.490 |
Rmerge | 0.287 | 0.726 |
Number of reflections | 9977 | 2380 |
<I/σ(I)> | 6.5 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9 | 8.8 |
CC(1/2) | 0.987 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M Tris pH7.0, 20% w/v PEG 1000 |