8EBM

Structure of KLHDC2 substrate binding domain bound to KLHDC2's C-degron mimic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	44.761, 88.634, 89.082
Unit cell angles	90.00, 104.57, 90.00

Refinement procedure

Resolution	39.410 - 1.580
R-factor	0.1946
Rwork	0.192
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6do2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.640
High resolution limit [Å]	1.580	3.400	1.580
Rmerge	0.070	0.043	0.644
Rmeas	0.084	0.052	0.766
Rpim	0.046	0.030	0.410
Total number of observations	296998
Number of reflections	90061	8910	8993
<I/σ(I)>	11.1
Completeness [%]	97.8	95.4	98
Redundancy	3.3	3	3.3
CC(1/2)		0.994	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	8-12% PEG3350, 0.2M NaSCN, 0.1 M Bis-Tris Propane, 5 nM Hexamine cobalt (III) chloride, pH = 7.5