8E3G

BMP2/GDF5 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-04-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	98.321, 98.321, 174.783
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.550 - 2.800
R-factor	0.1951
Rwork	0.193
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1waq 1reu
RMSD bond length	0.015
RMSD bond angle	1.336
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.600	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.173	1.363
Rpim	0.055	0.426
Number of reflections	24734	2428
<I/σ(I)>	11.2	2.3
Completeness [%]	100.0
Redundancy	11	11.2
CC(1/2)	0.995	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris, magnesium formate