8E1P
Crystal structure of BG505 SOSIP.v4.1-GT1.2 trimer in complex with gl-PGV20 and PGT124 Fabs
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 146.057, 157.666, 158.558 |
Unit cell angles | 90.00, 102.97, 90.00 |
Refinement procedure
Resolution | 49.760 - 3.820 |
R-factor | 0.2723 |
Rwork | 0.271 |
R-free | 0.30110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w6d |
RMSD bond length | 0.003 |
RMSD bond angle | 0.531 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.760 | 3.880 |
High resolution limit [Å] | 3.800 | 3.820 |
Number of reflections | 63948 | 5699 |
<I/σ(I)> | 4.3 | |
Completeness [%] | 95.7 | |
Redundancy | 2.9 | |
CC(1/2) | 0.780 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M ammonium sulfate, 0.1 M Tris, pH 8.5, 12% w/v PEG8000 |