8DYE

Crystal structure of human SDHA-SDHAF4 assembly intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-06-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 2
Unit cell lengths	139.049, 69.299, 72.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.570 - 1.440
R-factor	0.1559
Rwork	0.155
R-free	0.17280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vax 3sfd
RMSD bond length	0.008
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.440	1.450
Rmerge	0.090	1.390
Number of reflections	125714	6190
<I/σ(I)>	35.61	1.53
Completeness [%]	99.8	100
Redundancy	7
CC(1/2)	0.993	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	298	100 mM Sodium malonate, 12 % w/v PEG 3350, 1 mM DTT