8DYD
Crystal structure of human SDHA-SDHAF2-SDHAF4 assembly intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.048, 103.206, 126.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.490 - 1.520 |
| R-factor | 0.1581 |
| Rwork | 0.157 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vax 3sfd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.081 | |
| Rpim | 0.041 | 0.491 |
| Number of reflections | 122221 | 11699 |
| <I/σ(I)> | 24.4 | 1.7 |
| Completeness [%] | 91.7 | 89 |
| Redundancy | 4.2 | 4.1 |
| CC(1/2) | 0.904 | 0.648 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 200 mM Ammonium citrate tribasic pH 7.0, 20% w/v Polyethylene glycol 3,350 |
