8DYD

Crystal structure of human SDHA-SDHAF2-SDHAF4 assembly intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-08-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	66.048, 103.206, 126.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.490 - 1.520
R-factor	0.1581
Rwork	0.157
R-free	0.18420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vax 3sfd
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.520	1.520
Rmerge	0.081
Rpim	0.041	0.491
Number of reflections	122221	11699
<I/σ(I)>	24.4	1.7
Completeness [%]	91.7	89
Redundancy	4.2	4.1
CC(1/2)	0.904	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	200 mM Ammonium citrate tribasic pH 7.0, 20% w/v Polyethylene glycol 3,350