8DY5

Crystal Structure of spFv CAT2200 LH in complex with IL-17A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	82.430, 226.380, 75.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.250 - 2.200
R-factor	0.1902
Rwork	0.188
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qhu
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.260	45.260	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.120	0.032	1.012
Rmeas	0.134	0.037	1.127
Number of reflections	36090	452	2630
<I/σ(I)>	10.27	30.33	1.98
Completeness [%]	99.4	95	99.5
Redundancy	5
CC(1/2)	0.997	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	400 mM LiSO4, 15.5% PEG 3350