8DSF
Structure of cIAP1 with BCCov
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.990, 30.770, 118.630 |
| Unit cell angles | 96.70, 90.22, 111.08 |
Refinement procedure
| Resolution | 28.520 - 1.500 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kmn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.850 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.519 | 1.495 |
| High resolution limit [Å] | 1.391 | 1.391 |
| Rpim | 0.037 | 0.428 |
| Number of reflections | 47320 | 2367 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 72.4 | 27.3 |
| Redundancy | 1.8 | |
| CC(1/2) | 0.998 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.17 M Sodium Acetate, 0.085 M TrisBase pH 8.5, 25.5% w/v PEG 4000, 15% v/v glycerol |
